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Vol.48, No.6, 2014
Current Issue
Archive (2009~
Archive-the Korean Journal of Pathology(1967~2008)
Archive-the Korean Journal of Cytopathology(1990~2008)
Most Read Articles
  Vol.43, No.5:420-427, October 2009
Original articles
http://dx.doi.org/10.4132/KoreanJPathol.2009.43.5.420
DNA Methylation Profiles of MGMT, DAPK1, hMLH1, CDH1, SHP1, and HIC1 in B-Cell Lymphomas

Sung Sun Kim, Young Hyo Choi, Chang Woo Han, Yoo Duk Choi, Youngkyu Park, Je Jung Lee, Hyeoung Joon Kim, Il Kwon Lee, Ji Shin Lee, Sang Woo Juhng, Chan Choi

Department of Pathology, Chonnam National University Hwasun Hospital

Background : This study was designed to examine the prevalence of aberrant promoter methylation
in a selected panel of genes potentially involved in lymphoid tumors.

Methods : The
promoter hypermethylation status of MGMT, DAPK1, hMLH1, CDH1, SHP1, and HIC1 was
measured by methylation-specific PCR for 82 cases of B-cell lymphoma. Immunohistochemical
staining using MGMT and SHP1 antibodies was conducted on 43 out of 82 cases.


Results : The number of MGMT aberrant methylations was lower in diffuse large B-cell lymphoma
(DLBCL) than in other malignant lymphomas. The methylation of DAPK1 was frequently
detected in follicular lymphoma (FL), marginal zone B-cell lymphoma (MZL) and
DLBCL. With one exception, methylation of hMLH1 was not observed in B-cell lymphomas.
The methylation frequency of CDH1, and HIC1 was similar in B-cell lymphomas. However,
the methylation of SHP1 gene was more frequently observed in cases of FL, DLBCL, and
MZL than in chronic lymphocytic lymphoma. MGMT and SHP1 promoter methylation were
inversely correlated with the protein expression observed upon immunohistochemical staining.


Conclusions : Aberrant promoter methylation of multiple genes occurs with variable frequency
throughout the B-cell lymphomas, and methylation of hMLH1 is rarely observed in B-cell
lymphomas.
Key Words : Lymphoma; Methylation; B-lymphocytes

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