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Vol.48, No.6, 2014
Current Issue
Archive (2009~
Archive-the Korean Journal of Pathology(1967~2008)
Archive-the Korean Journal of Cytopathology(1990~2008)
Most Read Articles
  Vol.43, No.5:400-407, October 2009
Original articles
The Loss of E-cadherin is Associated with the Epigenetic Alteration of CDH1 in Breast Cancer and it is also Associated with an Abnormal β-catenin Expression in Lobular Carcinoma

Gwangil Kim, Ji Young Kim, Hee Jung An, Haeyoun Kang, Tae Heon Kim, Jung Yon Shim, Jin Hyung Heo, Hai Lin Park, Young Kil Choi

Department of Pathology, Bundang CHA Medical Cente

Background : APC and E-cadherin are the key molecules in the Wnt/β-catenin pathway. We
attempted to define the epigenetic alteration of APC and CDH1 (the E-cadherin gene) and
the expression of Wnt-related molecules in human mammary carcinomas.

Methods : Sixtyfour
mammary carcinomas, including 52 invasive ductal carcinomas (IDCs) and 12 invasive
lobular carcinomas (ILCs), were evaluated using methylation-specific PCR and immunohistochemistry.
We performed immunohistochemistry for E-cadherin, β-catenin, APC, Wnt1, cyclin
D1, ER, PR and C-erb B2.

Results : Hypermethylation of APC and CDH1 was observed in
38 (59%) and 28 (44%) cases, respectively. CDH1 hypermethylation in ILCs was increased
compared to that in IDCs (p=0.002) and it was associated with the loss of E-cadherin (p=0.02)
and β-catenin (p=0.042). APC methylation was positively correlated with the ER expression
(p=0.021). Abnormal cytoplasmic localization of β-catenin was found in 10 cases and any expression
was not detected in six cases. In ILCs, the E-cadherin or β-catenin expression was
markedly decreased compared to that in IDCs (p<0.001 in both).

Conclusions : Methylation
of APC or CDH1 was relatively frequent in mammary carcinomas. The loss of E-cadherin in
mammary carcinoma was associated with CDH1 methylation, and abnormal β-catenin expression
was related to the loss of E-cadherin in ILC.
Key Words : Breast neoplasms; Wnt1 protein; Beta catenin; DNA methylation; E-cadherin

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