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Vol.48, No.6, 2014
Current Issue
Archive (2009~
Archive-the Korean Journal of Pathology(1967~2008)
Archive-the Korean Journal of Cytopathology(1990~2008)
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  Vol.43, No.5:387-392, October 2009
Molecular Biologic Techniques in Cytopathologic Diagnosis

Tae Sook Hwang

Department of Pathology, Konkuk University Medical Center, 4-12 Hwayang-dong, Gwangjin-gu, Seoul 143-729, Korea

Molecular diagnosis is an application of the knowledge on molecular mechanisms of disease
to diagnosis, therapeutic decision-making, and prognostication. Basically any molecular diagnostic
technique could be used in molecular diagnostic cytopathology. Currently applicable
molecular techniques in the cytopathology field include PCR based molecular techniques
(SSCP, DHPLC, RFLP, LOH, MSI, RT-PCR, QRT-PCR, allele-specific PCR, sequencing, and
methylation analysis), FISH, cDNA microarray, aCGH, and reverse-phase protein microarray,
etc. Exfoliative cytology as well as fine needle aspiration cytology specimen can be used for
analysis. In order to obtain a successful result, collection of target cells without contamination
of the blood cells, inflammatory cells including histiocytes, and stromal cells, and a good DNA
yield are most important. Molecular diagosis finds its full meaning when interpreted by those
who can combine the clinical background of the disease with morphological, immunocytochemical,
and molecular diagnostic results. Therefore, these assays would fulfill their full potential
when interpreted by the cytopathologists.
Key Words : Molecular biologic technique; Cytopathologic diagnosis

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